Research Project Summary

This summer I set out to better understand how the two-component signal transduction (TCST) system crdRS regulates the napA gene in the bacterium Helicobacter pylori. TCST systems serve as important processes for bacteria to respond to environmental stimuli, and studying these systems will allow us to better understand how a bacterium survives in its particular […]

Monroe Update 3

I ended my last blog post describing the difficulty of inserting the cat gene into my region of interest. Since those initial methods were unsuccessful, I had to try a new approach. In order to generate new samples of the cat gene to work with, I grew up E. coli that contained plasmid pBSC103, a […]

Monroe Update 2

BglII

It’s been awhile since my last post! I ended my last post describing the cloning reaction that allowed me to make a large number of copies of the plasmid that contained the region of interest. I had to then extract the plasmid from the bacteria grown up in the broth cultures using a procedure called […]