Abstract-Descriptive Analysis of Cells in Xenopus laevis Embryonic Central Nervous System during Secondary Neurogenesis

This project will be an analysis of the distribution and lineage of proliferative embryonic cells during secondary neurogenesis in the Central Nervous System (CNS) of the African Clawed Frog, Xenopus laevis. The mechanism of embryonic neurogenesis in Xenopus laevis is a major focus in developmental neurobiology. While the first wave of neurogenesis, known as primary […]

Final blog post: Summary and future directions

This summer, I investigated the plasticity of the anterior-posterior axis in the developing nervous system of Xenopus laevis.  In order to research this topic, I performed transplant experiments on embryos while they were undergoing gastrulation, which consisted of removing and rotating an embryo’s anterior-posterior neural axis 180 degrees. I then observed the embryos as they […]

Blog post 3: What’s a double in situ?

The second part of my project dealt with the analysis of the frog embryos after a transplantation experiment. Beyond looking at the obvious physical appearance of the embryos (mentioned in my first post), I wanted to see how transplantation at different stages might affect the expression of several regional marker genes. These are genes that […]