Concise Synthesis and Biological Testing of Natural Products with Potential Anticancer Activity

Quinones are oxidation products of aromatic compounds. Quinones and derivatives thereof are known to show inhibitory properties in the mitochondrial complex I in the mammalian respiratory system. They bind to the active site and inhibit the enzymatic behavior of the mitochondrial complex I. Because complex I is linked to cancer survival, inhibition thereof has been […]

GFP sensors 2: Resistance is centri-futile


For these past two weeks, I have been growing bacteria to express fluorescent protein so I can harvest it. The protein expression process takes about one week, and I am making four different variants for my experiments. Fortunately, they can be expressed simultaneously. Unfortunately, some of the bacteria cultures have been refusing to grow for some reason. This has […]

GFP sensors 1: Trick-or-titrate


This past week, I performed some titrations to finish up a set of data collection. Unlike titrations in high school that use a color-changing indicator, I was using a pH probe to determine when the titration was finished. As such, I had to be sure to calibrate the instrument properly. Every day it is used, the pH […]