Summer 2016 Summary

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This summer, I have been mainly working on three projects: secondary neurogenesis, transgenics using I-SceI meganuclease, and neural tube sidedness. They yielded many exciting results, and the following are the summaries. Secondary neurogenesis Goal: determine the localization of neuronal and neural progenitor cell population during secondary neurogenesis on a single cell resolution  Methodology: perform a […]

Research update 3

My research has had some exciting breakthroughs over the last few weeks. The first one is about secondary neurogenesis, which is my main project. The goal is to identify the localization and possibly colocalization of neuronal and neural progenitor cells during secondary neurogenesis on a single cell resolution. The double fluorescent in situ hybridization to […]

Update 2

Since my first update, my project has had quite a few breakthroughs. The most significant one is that I successfully performed a double fluorescent in situ hybridization (Sox2+NBT) plus immunocytochemistry (memRFP). Experience indicates that simply eliminating the proteinase K treatment step yields a much better signal than the EtOH/Xylene and cold Acetone treatment. These procedures were done on stage […]